The Queen beams towards the camera in an unseen portrait released ahead of the beloved monarch’s funeral tomorrow morning.
World leaders have descended on London ahead of the service at Westminster Abbey, with doors set to open at 8am amid heightened security in the capital.
The Queen’s lying-in-state period is set to end at 6.30am on Monday as emotional mourners continue to line up in a bid to see her coffin and pay tribute to her.
Among the guests are foreign royalty flying including Spain’s King Felipe and Queen Letizia, King Willem-Alexander and Queen Máxima of the Netherlands, King Harald V and Queen Sonja of Norway and Prince Albert II and Princess Charlene of Monaco.
Ahead of tomorrow’s sorrow-filled event a photo of the Queen showing off her radiant smile was released to the public.
The image was snapped in May by photographer Ranald Mackechnie ahead of the Platinum Jubilee celebrations in Windsor Castle.
In the touching image, the Queen matched her blue dress with her favourite three-strand pearl necklace, pearl earrings and her aquamarine and diamond clip brooches - an 18th birthday present from her father King George VI in 1944.
Eagle-eyed royal fans will recognise the Queen’s brooches from when she addressed the nation on the 75th anniversary of VE Day in 2020 and her Diamond Jubilee televised speech in 2012.
The two art deco-style pieces, worn one below the other, were made by Boucheron from baguette, oval and round diamonds and aquamarines.
In a televised tribute to her mother-in-law earlier this evening, the Queen Consort recalled the Queen's "wonderful blue eyes" and said: "I will always remember her smile."
Other royals set to attend tomorrow’s service will arrive from Greece, Sweden, Denmark and Belgium.
Joining them are the six surviving former British PMs who served under the Queen, along with Liz Truss.
Mourners for the queue were continuing to join earlier this afternoon, with the last now having taken their place among the mourners.
It comes as St John Ambulance revealed 259 people in the queue to see the Queen lying in State needed medical support on Saturday amid plunging temperatures.
So far, on Sunday around 98 people needed medical support, nine of whom needed to be taken to hospital.
Earlier tonight millions paused for a national moment of reflection
Big Ben tolled to mark the start of the minute’s silence and will also ring on Monday at one-minute intervals as the funeral procession departs the service.
A muffler will be used for the bell, which reduces the impact of the sound and creates a quieter tone.