A soldier collapsed and at least two military members were helped away at the end of the Queen's coffin procession.
The trio required assistance after getting into difficulty at Wellington Arch, where the monarch's coffin was transferred from a gun carriage to the state hearse.
One soldier collapsed to the ground after Her Majesty departed.
Meanwhile, two others were seen being helped away before and after the transfer of the casket.
Her Majesty is now being carried to Windsor, where she will finally be buried.
It comes after a police officer fainted while on duty in London for the Queen's funeral.
The officer collapsed shortly before the procession came down The Mall.
He was carried away on a stretcher during the event.
Medics rushed him away minutes before the Queen’s coffin was carried past.
His condition remains unconfirmed but is not thought to be serious.
A Royal guard also fainted and fell off the podium where he was protecting the Queen's coffin on Thursday.
There were gasps from mourners as he fell to the ground which was caught in a video.
It happened as a set of guards were beginning to swap duties and one appeared to be trembling before seconds later falling.
Two police officers could be seen racing over to pick him up before the video cut out and then went to images of the Houses of Parliament.
Shocked viewers also took to social media with one tweeting: "He must've been so overwhelmed with what was happening."
Another said: "Bless him - just happened to tune in; saw he was a bit wobbly and feared he'd faint. Hope he's ok."
Hundreds of people needed medical attention while queuing to see the Queen lying-in-state on Saturday, with St John's Ambulance Service confirming at least 259 people received help from their staff.
As night time temperatures dropped as low as 4C, the London Ambulance Service was kept busy providing medical help to those lining up or visiting the area to pay their respects.
In total, hundreds of mourners in the crowds have needed help so far.
Around 1,000 St John Ambulance volunteers will be on duty during the state funeral in London and Windsor, including 800 who will be on hand to provide medical and first aid support.