An emotional Prince Harry appeared to lose his composure during a service at Westminster Hall after the Queen's coffin procession from Buckingham Palace, as he was seen wiping his eyes.
And the Prince was not the only royal to temporarily abandon the traditional British stiff upper lip during the ceremony.
The coffin carrying the late Queen Elizabeth II left Buckingham Palace for the final time on Wednesday afternoon and was moved to Westminster Hall.
Here, it will lie-in-state for members of the public to pay their respects until Monday 19 September, the day of the late monarch's funeral.
The Queen's coffin was followed on foot by her four children, King Charles III, 73, Princess Anne, 72, Prince Andrew, 62, and Prince Edward, 58, as well as her grandsons, Prince William, 40, and Prince Harry, 37, - who walked side-by-side - and Peter Phillips, 44, who is the son of Princess Anne.
Follow the latest updates as the world mourns Queen Elizabeth II and King Charles III begins his reign in our live blog.
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Members of the royal family, including King Charles, Prince William, Prince Harry and their wives, Camila Queen Consort, Kate Middleton and Meghan Markle, then attended a service for the reception of Her Majesty the Queen's coffin at Westminster Hall before the lying-in-state begins.
During the service, Prince Harry was seen wiping his eyes during the emotional moment.
Prince Edward and Sophie, Countess of Wessex's daughter, Lady Louise Windsor, was seen wiping away tears during the service, as was Princess Eugenie of York.
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In another touching moment, Prince William was seen comforting a sombre-looking Katherine, Princess of Wales, as he placed a hand on her back.
While King Charles III appeared to have an emotional moment prior to the ceremony, and was seen glassy-eyed during it.
Meghan, who travelled by car alongside the Countess of Wessex during the procession, looked to be wiping a tear from her eye after the ceremony at Westminster Hall, when she left in a car with Prince Harry.
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The procession arrived at Westminster Hall at 3pm, when the Queen's coffin was placed on a purple-covered catafalque.
A 20-minute service led by the Archbishop of Canterbury and the Dean of Westminster began soon after.
During the service, members of the Royal Family stood in formation facing the coffin, which was flanked with a tall, yellow flickering candle at each corner of the wide scarlet platform.
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The King and Queen Consort stood at the front, a metre or so apart, with the Princess Royal and Vice Admiral Sir Tim Laurence behind them.
The Duke of York was alone in the next row, with the Earl and Countess of Wessex behind him.
The Queen's grandchildren followed - Prince William and his wife Kate - the new Prince and Princess of Wales - and Harry and Meghan.
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Her Majesty will now lie in state in the hall until Monday, when her funeral will take place.
Brits have already started queuing to view the monarch's coffin.
Thousands of people are expected to visit London to see the Queen lying-in-state between now and her funeral on Monday.
You can leave your tributes to Queen Elizabeth II here.