The last mourner to visit the Queen lying in state said she was very privileged as the viewing of the coffin at Westminster Hall closed to the public after more than four days.
Chrissy Heerey, a serving member of the RAF from Melton Mowbray, had queued up twice overnight to pay her respects.
Ms Heerey said: "I was the last person to pay my respects to the Queen and it felt like a real privilege to do that.
"I'd already been round once, I went in at 1.15 this morning.
"It's one of the highlights of my life and I feel very privileged to be here."
Queen Elizabeth II had been lying in state since last Wednesday and saw thousands of people queuing up in queue that surpassed five miles in length at its peak.
Some mourners and well-wishers had to wait up to 25 hours in line, such was the demand to for people paying their respects.
The Department for Digital, Culture, Media and Sport (DCMCS) confirmed the queue had finally closed at around 10.45pm on Sunday night.
The DCMS tweeted: "The queue to attend Her Majesty The Queen's Lying-in-State is at final capacity and is now closed to new entrants.
"Please do not attempt to join the queue Stewards will manage those already nearby Thank you for your understanding".
The funeral for the country's longest-serving monarch is due to start on Monday at 11am.
Just before then, at around 10:44am the coffin will be led in procession from Westminster Hall a few few metres away to Westminster Abbey where the service will begin.
King Charles III will follow behind the coffin along with the late Queen's three other children, Princess Anne, Prince Andrew, Prince Edward.
Other royals in the procession towards the abbey include the Prince of Wales, Prince Harry and the remaining grandchildren.
Around 2,000 world leaders will attend the service, including US President Joe Biden and Japan’s Emperor Naruhito and Empress Masako.
A two minute silence will be carried out at the end of the funeral around 11:55am before the Queen’s coffin will be then taken from the abbey on to its final resting place.
The late monarch will be buried next to her husband Prince Philip, Duke of Edinburgh in the royal vault of St George's Chapel in Windsor.
A private ceremony will be held there on Monday evening in the King George VI memorial with senior royals in attendance.