Berlin (AFP) - Eintracht Frankfurt lost 2-1 at home to Freiburg on Sunday in a final tune-up before their crunch Europa League quarter-final at Spanish giants Barcelona.
Frankfurt striker Filip Kostic equalised after Vincenzo Grifo gave Freiburg an early lead before Nils Petersen headed in the visitors' late winner.
It was a disappointing result for Frankfurt, four days before their Europa League quarter-final, second leg at Barcelona's Camp Nou having drawn the home tie 1-1 last Thursday.
"We are brutally disappointed with the result," admitted Frankfurt coach Oliver Glasner.
"It took us 15 to 20 minutes to shake off Thursday's game.
"I have a lot of respect for the performance the boys then delivered.
"Freiburg won because of their efficiency, but we won't let the unfortunate defeat get us down."
Sunday's league win is a significant boost for Freiburg in their bid to reach the Champions League next season.
With five games left, they drew level on 48 points with fourth-placed RB Leipizg with the top four clubs qualifying for the Champions League.
There was a brief delay at the start of the first half when two environmental protestors each tied themselves to a post in the Frankfurt goal.
After Kostic's equaliser ruled out Grifo's opening goal, Petersen came off the bench to head in the winner from a free-kick on 69 minutes.
Frankfurt thought they had equalised again when Japan midfielder Daichi Kamada hit the bottom corner late on, but VAR ruled he was offside.
Minutes before, Frankfurt replacement forward Ajdin Hrustic had hit the post as the hosts pushed for a second goal.
Earlier, a slip while taking a penalty cost France winger Moussa Diaby the chance to score as Bayer Leverkusen were held to a goalless draw at Bochum.
Leverkusen, who remain third, were awarded a penalty when Czech Republic striker Patrik Schick went down in the area midway through the second half.
Diaby stepped up to take the spot kick, but slipped striking the ball, which flew into the net only after rebounding off his standing leg.
The referee spotted the second touch and disallowed the goal.
"It was just unlucky", Leverkusen captain Lukas Hradecky said of Diaby's miss, "but we hope that will be an important point."
The result leaves Leverkusen trailing 17 points behind runaway league leaders Bayern Munich, who inched towards a tenth straight Bundesliga title on Saturday with a 1-0 home win over Augsburg.