The cold and wet conditions meant that most teams limited their running during the 105-minute session ahead, which is expected to be dry.
Albuquerque set the quickest time at the wheel of the #10 WTR Acura of 1m48.730s 38 minutes from the end of the session, going 0.733s quicker than the #01 Chip Ganassi Racing Cadillac DPi-V.R driven by reigning IndyCar champion Alex Palou.
Tom Blomqvist had put the #60 Meyer Shank Racing Acura on top early on, but the Anglo-Swedish driver's effort of 1m49.583s still proved good enough for third place.
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Fourth overall was the best of the LMP2 runners, with Formula 2 veteran Luca Ghiotto leaving it late to post the quickest time in the class of 1m49.597s aboard the #69 G-Drive Racing by APR ORECA.
That was enough to beat the Tower Motorsport ORECA of Rui Andrade by over two seconds, while IndyCar ace Patricio O'Ward was third in class aboard the #81 DragonSpeed entry that had gone fastest in FP1.
Best of the remaining DPi runners was the #5 JDC-Miller MotorSports Cadillac of Tristan Vautier, who was eighth overall behind the #68 G-Drive LMP2 car driven by DTM star Rene Rast.
The #02 Ganassi Cadillac and the two AXR-run cars, the #31 Whelen Engineering Caddy and the FP2 pace-setting #48 Ally machine, did not set laptimes.
In LMP3, Ugo de Wilde set the pace aboard the #26 Muehlner Motorsports Duqueine with a best effort of 1m56.933s - some 1.7s ahead of the next-best car in the class, the Sean Creech Motorsport Ligier of Seb Priaulx.
GTD Pro was topped by the #97 Proton-run WeatherTech Racing Mercedes-AMG GT3, with Daniel Juncadella posting the fastest time of 1m58.431s.
Another Mercedes was fastest in the Pro-Am GTD class, the SunEnergy1 car of Raffaele Marciello, a further 0.148s back and narrowly ahead of the second-best GTD Pro car, the Pfaff Motorsports Porsche 911 GT3 R driven by Matt Campbell.
Third in GTD Pro was Andrea Caldarelli in the #63 TR3 Racing Lamborghini Huracan GT3, followed by Corvette Racing newcomer Marco Sorensen in the #4 C8.R and Matteo Cairoli in the #79 WeatherTech Porsche.
Franck Perera was second-fastest in GTD aboard the T3 Motorsport Lambo and Daniel Morad in the Alegra Motorsports Mercedes.
The final practice session for the 2022 Daytona 24 Hours takes place on Friday at 11.15am local time.
Practice results:
| Cla | Num | Driver | Class | Chassis | Time | Gap |
|---|---|---|---|---|---|---|
| 1 | 10 |
 Ricky Taylor  Filipe Albuquerque Alexander Rossi  Will Stevens
| DPi | Acura DPi | 1'48.730 | |
| 2 | 01 |
 Renger van der Zande  Sébastien Bourdais  Scott Dixon  Alex Palou
| DPi | Cadillac DPi | 1'49.463 | 0.733 |
| 3 | 60 |
Oliver Jarvis Tom Blomqvist  Helio Castroneves Simon Pagenaud
| DPi | Acura DPi | 1'49.583 | 0.853 |
| 4 | 69 |
John Falb  James Allen  Luca Ghiotto Tijmen van | LMP2 | ORECA LMP2 07 | 1'49.597 | 0.867 |
| 5 | 8 |
 John Farano  Louis Deletraz Rui Pinto  Ferdinand Habsburg
| LMP2 | ORECA LMP2 07 | 1'51.782 | 3.052 |
| 6 | 81 |
Eric Lux Devlin DeFrancesco  Patricio O'Ward Colton Herta
| LMP2 | ORECA LMP2 07 | 1'51.833 | 3.103 |
| 7 | 68 |
François Heriau Oliver Rasmussen  Ed Jones  René Rast
| LMP2 | ORECA LMP2 07 | 1'52.301 | 3.571 |
| 8 | 5 |
Tristan Vautier Richard Westbrook Loic Duval Ben Keating
| DPi | Cadillac DPi | 1'52.793 | 4.063 |
| 9 | 20 |
 Dennis Andersen Anders Fjordbach Fabio Scherer Nico Mueller | LMP2 | ORECA LMP2 07 | 1'55.137 | 6.407 |
| 10 | 22 |
Jim McGuire Guy Smith Philip Hanson Will Owen
| LMP2 | ORECA LMP2 07 | 1'56.820 | 8.090 |
| 11 | 26 |
Nolan Siegel Cameron Shields Charles Crews Ugo De | LMP3 | Duqueine D08 | 1'56.933 | 8.203 |
| 12 | 18 |
Dwight Merriman Kyle Tilley Ryan Dalziel Paul-Loup Chatin
| LMP2 | ORECA LMP2 07 | 1'57.184 | 8.454 |
| 13 | 97 |
Cooper MacNeil Daniel Juncadella Maro Engel Jules Gounon
| GTD PRO | Mercedes-AMG GT3 | 1'58.431 | 9.701 |
| 14 | 75 |
Kenny Habul Luca Stolz Raffaele Marciello Fabian Schiller
| GTD | Mercedes-AMG GT3 | 1'58.579 | 9.849 |
| 15 | 9 |
Matt Campbell Mathieu Jaminet Felipe Nasr
| GTD PRO | Porsche 911 GT3 R | 1'58.699 | 9.969 |
| 16 | 71 |
Franck Perera LLarena Mateo Paul Maximilian Mikhail Goikhberg
| GTD | Lamborghini Huracan GT3 | 1'58.812 | 10.082 |
| 17 | 33 |
Joao Barbosa Malthe Jakobsen Seb Priaulx Lance Willsey
| LMP3 | Ligier JS P320 | 1'58.836 | 10.106 |
| 18 | 63 |
Marco Mapelli Andrea Caldarelli Mirko Bortolotti Rolf Ineichen
| GTD PRO | Lamborghini Huracan GT3 | 1'58.943 | 10.213 |
| 19 | 28 |
Michael de Quesada Daniel Morad Linus Lundqvist Maximillian Goetz | GTD | Mercedes-AMG GT3 | 1'59.177 | 10.447 |
| 20 | 4 |
Tommy Milner Nick Tandy Marco Sorensen
| GTD PRO | Corvette C8.R GTD | 1'59.199 | 10.469 |
| 21 | 79 |
Cooper MacNeil Julien Andlauer Matteo Cairoli  Alessio Picariello
| GTD PRO | Porsche 911 GT3 R | 1'59.746 | 11.016 |
| 22 | 52 |
Ben Keating Mikkel Jensen Scott Huffaker Nicolas Lapierre
| LMP2 | ORECA LMP2 07 | 2'00.000 | 11.270 |
| 23 | 3 |
Antonio Garcia Jordan Taylor Nick Catsburg
| GTD PRO | Corvette C8.R GTD | 2'00.132 | 11.402 |
| 24 | 6 |
Efrin Castro Moritz Kranz Ori Ayrton Joel Miller
| LMP3 | Duqueine D08 | 2'00.229 | 11.499 |
| 25 | 15 |
Dirk Muller Patrick Assenheimer Austin Cindric
| GTD PRO | Mercedes-AMG GT3 | 2'00.251 | 11.521 |
| 26 | 70 |
Brendan Iribe Frederik Schandorff Ollie Millroy  Jordan Lee Pepper
| GTD | McLaren 720S GT3 | 2'00.305 | 11.575 |
| 27 | 39 |
Robert Megennis Corey Lewis Sandy Mitchell Jeff Westphal
| GTD | Lamborghini Huracan GT3 | 2'00.718 | 11.988 |
| 28 | 57 |
Russell Ward Philip Ellis Mikael Grenier Lucas Auer
| GTD | Mercedes-AMG GT3 | 2'01.102 | 12.372 |
| 29 | 36 |
Jarett Andretti Josh Burdon  Rasmus Lindh  Gabby Chaves
| LMP3 | Ligier JS P320 | 2'01.254 | 12.524 |
| 30 | 44 |
John Potter Andy Lally Spencer Pumpelly Jonathan Adam
| GTD | Aston Martin Vantage GT3 | 2'01.537 | 12.807 |
| 31 | 13 |
Orey Fidani Kuno Wittmer Lars Kern Matthew Bell
| LMP3 | Duqueine D08 | 2'01.543 | 12.813 |
| 32 | 74 |
Gar Robinson Felipe Fraga Kay van Berlo Michael Cooper
| LMP3 | Ligier JS P320 | 2'01.754 | 13.024 |
| 33 | 24 |
Philipp Eng Marco Wittmann Nick Yelloly Sheldon Van Der Linde
| GTD PRO | BMW M4 GT3 | 2'01.842 | 13.112 |
| 34 | 47 |
Roberto Lacorte Giorgio Sernagiotto Antonio Fuoco Alessio Rovera
| GTD | Ferrari 488 GT3 | 2'01.926 | 13.196 |
| 35 | 21 |
Mann Simon  Luis Perez Companc Nicklas Nielsen  Toni Vilander
| GTD | Ferrari 488 GT3 | 2'01.938 | 13.208 |
| 36 | 7 |
Mark Kvamme Austin McCusker Trenton Estep Antoine Doquin | LMP3 | Duqueine D08 | 2'01.964 | 13.234 |
| 37 | 96 |
Robby Foley Bill Auberlen Michael Dinan Jens Klingmann
| GTD | BMW M4 GT3 | 2'02.197 | 13.467 |
| 38 | 19 |
Bill Sweedler John Megrue Giacomo Altoè Jeff Segal
| GTD | Lamborghini Huracan GT3 | 2'02.600 | 13.870 |
| 39 | 11 |
Thomas Steven Josh Pierson Jonathan Bomarito Harry Tincknell
| LMP2 | ORECA LMP2 07 | 2'02.759 | 14.029 |
| 40 | 12 |
Frankie Montecalvo Aaron Telitz Richard Heistand Townsend Bell
| GTD | Lexus RC F GT3 | 2'02.849 | 14.119 |
| 41 | 14 |
Jack Hawksworth Ben Barnicoat Kyle Kirkwood
| GTD PRO | Lexus RC F GT3 | 2'02.939 | 14.209 |
| 42 | 99 |
Rob Ferriol Katherine Legge Stefan Wilson Nicholas Boulle
| GTD | Porsche 911 GT3 R | 2'03.538 | 14.808 |
| 43 | 59 |
Lance Bergstein Jon Miller Patrick Gallagher Paul Holton
| GTD | McLaren 720S GT3 | 2'03.832 | 15.102 |
| 44 | 98 |
Paul Dalla Lana David Pittard Charlie Eastwood Nicki Thiim
| GTD | Aston Martin Vantage GT3 | 2'03.890 | 15.160 |
| 45 | 62 |
Alessandro Pier Guidi James Calado Daniel Serra Davide Rigon
| GTD PRO | Ferrari 488 GT3 | 2'04.544 | 15.814 |
| 46 | 54 |
Jon Bennett Colin Braun George Kurtz Nic Jönsson
| LMP3 | Ligier JS P320 | 2'05.269 | 16.539 |
| 47 | 42 |
Don Yount Benja Hites Jaden Conwright Markus Palttala
| GTD | Lamborghini Huracan GT3 | 2'05.790 | 17.060 |
| 48 | 38 |
Dan Goldburg Hikaru Abe Garett Grist Nicolas Pino | LMP3 | Ligier JS P320 | 2'05.800 | 17.070 |
| 49 | 66 |
Kyffin Simpson Till Bechtolsheimer Marc Miller Mario Farnbacher
| GTD | Acura NSX GT3 | 2'05.845 | 17.115 |
| 50 | 32 |
Mike Skeen Guy Cosmo Stevan McAleer Scott Andrews
| GTD | Mercedes-AMG GT3 | 2'06.214 | 17.484 |
| 51 | 64 |
Ted Giovanis Hugh Plumb Matt Plumb Owen Trinkler
| GTD | Porsche 911 GT3 R | 2'07.382 | 18.652 |
| 52 | 29 |
Frits van Eerd Giedo van der Garde Murry Dylan Rinus van Kalmthout
| LMP2 | ORECA LMP2 07 | 2'09.037 | 20.307 |
| 53 | 16 |
Ryan Hardwick Zacharie Robichon Jan Heylen Richard Lietz
| GTD | Porsche 911 GT3 R | 2'09.678 | 20.948 |
| 54 | 34 |
Kyle Washington James Sofronas Jeroen Bleekemolen Klaus Bachler
| GTD | Porsche 911 GT3 R | 2'09.925 | 21.195 |
| 55 | 2 |
Laurens Vanthoor Patrick Pilet  Dennis Olsen Alexandre Imperatori
| GTD PRO | Porsche 911 GT3 R | 0.000 | |
| 56 | 02 |
Earl Bamber Alex Lynn Marcus Ericsson Kevin Magnussen
| DPi | Cadillac DPi | 0.000 | |
| 57 | 23 |
Ross Gunn Alex Riberas Maxime Martin
| GTD PRO | Aston Martin Vantage GT3 | 0.000 | |
| 58 | 25 |
Connor de Phillippi John Edwards Augusto Farfus Jesse Krohn
| GTD PRO | BMW M4 GT3 | 0.000 | |
| 59 | 27 |
Roman De Angelis Ian James Darren Turner Tom Gamble | GTD | Aston Martin Vantage GT3 | 0.000 | |
| 60 | 31 |
Pipo Derani Tristan Nunez Mike Conway
| DPi | Cadillac DPi | 0.000 | |
| 61 | 48 |
Mike Rockenfeller  Kamui Kobayashi Jimmie Johnson Jose Maria Lopez
| DPi | Cadillac DPi | 0.000 |
