Inspiral will miss the QIPCO 1,000 Guineas and be prepared for a seasonal comeback at Royal Ascot.
The bet365 Fillies' Mile winner, trained by John and Thady Gosden for owners Cheveley Park Stud, had been the long time favourite for the Classic.
But after some concern was expressed about her interrupted preparation on Friday, Gosden Snr today confirmed the daughter of Frankel will miss next Sunday's race.
"Having consulted both Mrs Thompson and Chris Richardson, a decision has been made not to run Inspiral in the 1,000 Guineas," he told the Racing Post.
"She missed some days training at a critical stage of her preparation and although she is well in herself now, we do not have her at the correct pitch to run in the Guineas.
"The focus now will be on the Coronation Stakes at Royal Ascot with probably a run beforehand. Obviously we had to make the right decision for the filly."
Inspiral is unbeaten in four races including the Group Two May Hill Stakes and the Group One bet365 Fillies' Mile in October.
The Aidan O'Brien-trained Tenebrism is the new favourite for the 1,000 Guineas at 11-4 with Betfair, with Dermot Weld's Homeless Songs at 11-2 and Jess Harrington's Discoveries on 6-1.
French raider Malavath is 7-1 while James Ferguson's Mise En Scene is now the shortest of the British-based contenders at 10-1 and Cachet at 14-1.
Betfair spokesman, Barry Orr said: ‘’Prior to confirmation this morning that Inspiral wouldn’t be taking up this engagement the market had already been shifting and Tenebrism was favourite but she has obviously hardened at the head of the betting and is sure to go off favourite this day week.
The 1000 Guineas – Betfair Betting: 11-4 Tenebrism, 11-2 Homeless Songs, 6-1 Discoveries, 7-1 Malavath, 8-1 Tuesday, 9-1 Zellie, 10-1 Mise En Scene, 14-1 Cachet, 16-1 Sandrine, 16-1 Wild Beauty, 20-1 bar.
