Paul Rusesabagina, who was declared a hero by the international community for shielding hundreds of Tutsis from the 1994 genocide in his hotel, is alleged to have sent money from the US to the Democratic Forces for the Liberation of Rwanda (FDLR), a Hutu-majority rebel group whose commanders were implicated in the killings.
Rusesabagina, who was played by Don Cheadle in the film Hotel Rwanda, insists he has not sent money to Rwanda for at least seven years and dismissed the allegations as part of a smear campaign against him because he opposed the government of Paul Kagame, the Rwandan president, in the past.
Martin Ngoga, Rwanda's most senior prosecutor, claimed he had hard evidence that Rusesabagina, 56, sent money to two FDLR commanders in Burundi and Tanzania via the Western Union from San Antonio, Texas, where he has a home.
"Those who want to continue considering him as a hero can go on," Ngoga said. "We consider him a serious criminal suspect and we are challenging whoever speaks on his behalf to tell us whether he never sent money to these FDLR commanders we have in custody."
It is alleged that the money was designed to fund the recruiting of fighters for a new military wing of FDU-Inkingi, a Hutu opposition party headed by Victoire Ingabire.
Ingabire was remanded in custody earlier this month on charges of forming a terrorist group. Ngoga alleged that the FDLR commanders in custody have given evidence against both Ingabire and Rusesabagina.
Rusesabagina, who in 2005 was awarded the US Presidential Medal of Freedom, the country's highest civilian honour by former US President George W Bush, insists he has done nothing wrong.
"It is the latest step in a campaign against me by the Rwandan government that has included public insults, lies and physical harassment," he said.
"My foundation is advocating for a truth, justice and reconciliation process to try to foster sustainable peace in Rwanda ... but anyone who opposes Kagame inside or outside the country is treated with this kind of harassment."
No formal charges have yet been filed against him.
More than 500,000 Rwandans, mostly ethnic Tutsis and moderate Hutus, were killed in Rwanda's genocide.
Kagame, an ethnic Tutsi, has tried to downplay the role of ethnicity in post-genocide Rwanda, and people in the country rarely refer to themselves as Hutu or Tutsi and can face charges for speaking publicly about ethnicity.
But he has been criticised by human rights groups who accuse his regime of iron-fisted control and of silencing opposition politicians and media outlets with arrests and attacks in the run up to August presidential election.